Aunt Mimi (Rio 2) as Grandma Spankenheimer – The duteteragonist of the film. Jake and Daphne's loving grandmother and Mr. Spankenheimer's mother who loves Christmas. Grandma is the owner of a general store in Cityville. Merchandise at the store includes ornaments, toys, and Grandma's infamous "Killer Fruitcake".
Tiago (Rio 2) as Jake Spankenheimer – The main protagonist of the film, he is Grandma and Grandpa's grandson and the son of Mr. and Mrs. Spankenheimer and Daphne's brother. Jake leads the search for Grandma when she mysteriously vanishes, and Grandma being hit by Santa's sleigh. A firm believer in Santa Claus, Jake proves his family wrong when he finds the real Santa Claus.
Nigel (Rio) as Cousin Mel – The main antagonist of the film. Cousin Mel is a money grubbing do-badder who wears too much jewelry and beats Grandpa at cards. Cousin Mel is out to sell the store to the wealthy Austin Bucks, and makes Santa Claus the fall guy in a lawsuit over Grandma's disappearance so she can get all his money. Instead of a female, he is a male.
Eduardo (Rio 2) as Grandpa Spankenheimer – Jake and Daphne's grandfather, Grandma's daffy husband and Mr. Spankenheimer's father. Grandpa accidentally gives Cousin Mel power of attourney, giving Cousin Mel the power to sell Spankenheimer's General Store to Austin Bucks.
Iago (Aladdin) as Austin Bucks – A wealthy young businessman who owns much of Cityville. Bucks want to purchase Grandma's general store, saying that it would be the perfect location for the Sleighmobile, a contraption that would deliver the gifts on Christmas Eve.
Santa Claus – A fat old man who delivers gifts around the world on Christmas Eve. Santa accidentally runs over Grandma with his reindeer-drawn sleigh, and takes her to his hospital at his North Pole workshop.
Carla (Rio 2) as Daphne Spankenheimer – Jake's sister, Grandma and Grandpa's granddaughter and Mr. and Mrs. Spankenheimer's daughter.
Blu (Rio) as Mr. Spankenheimer – Jake and Daphne's father and Grandma and Grandpa's son.
Jewel (Rio) as Mrs. Spankenheimer – Jake and Daphne's mother and Grandma and Grandpa's daughter-in law.
Gabi (Rio 2) as I.M. Slime - The secondary antagonist of the film.
Nico (Rio) as Quincy - The tritagonist of the film.