- Dan Aykroyd as Yogi Bear, the picnic basket-stealing talking bear who lives in Jellystone Park.
- Justin Timberlake as Boo-Boo Bear, Yogi's sidekick, and sometimes the voice of reason.
- Tom Cavanagh as Ranger Smith, the head ranger of Jellystone Park.
- Katie Crown as Cindy Bear, the love-interest of Yogi Bear and a resident of Jellystone Park.
- Anna Faris as Rachel Johnson, a nature documentary filmmaker, and Ranger Smith's love interest. She is called "Miss Movie Lady" or "Miss Moviemaker Lady" by Yogi and Boo Boo. She has spent time with the animals that she makes a documentary on learning a gorilla's rage and pinning down people like a snow leopard.
- Bella Thorne as Selena Harvey, a college graduate.
- Ross Lynch as Bart Bouvier, Selena Harvey's love interest.